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Developmental Studies Hybridoma Bank
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R&D Systems
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Proteintech
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Proteintech
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Danaher Inc
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Affinity Biosciences
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Epitomics corp
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Santa Cruz Biotechnology
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Thermo Fisher
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Beyotime
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Novocastra
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Image Search Results
Journal: The Korean Journal of Internal Medicine
Article Title: Shen-Kang protects against tacrolimus-induced renal injury
doi: 10.3904/kjim.2017.276
Figure Lengend Snippet: Representative photomicrographs of (A) immunohistochemistry for osteopontin (OPN) (×200) and (B) immunoblotting of interleukin 17 (IL-17). VH, vehicle; SK, ShenKang; TAC, tacrolimus. a p < 0.05 vs. VH, b a p < 0.05 vs. TAC.
Article Snippet: Non-specific binding sites were blocked with normal horse serum diluted 1:10 in 0.3 % bovine serum albumin for 30 to 60 minutes, and then incubated for 2 hours at 4°C in
Techniques: Immunohistochemistry, Western Blot
Journal: bioRxiv
Article Title: Single-cell RNA sequencing identifies phenotypically, functionally, and anatomically distinct stromal niche populations in human bone marrow
doi: 10.1101/2022.01.26.477664
Figure Lengend Snippet: (A-D, F-G) UMAP (as in ) illustration of the normalized expression of selected ligand and receptor gene pairs. Red dashed lines in figures A, B and D mark the erythroid clusters. The blow-up shown in (G) is to better visualize PDGFA- and PDGFB-expressing cells. (E) Formalin-fixed, paraffin-embedded (FFPE) human BM slides were sequentially stained for DAPI (blue), CD45 (yellow), SPP1 (red), CD271 (pink), and NCAM1 (cyan) and scanned with an OlympusVS120 slide scanner. Lower panel: Single staining for each marker is shown as indicated under each image. Scale bars represent 50 µm. White lines indicate bone lining regions. b, Bone.
Article Snippet: For SPP1 staining, after deparaffinization, rehydration, and antigen retrieval, slides were permeabilized with 0.1% Triton X100 (Sigma Ultra, T9284) solution at 37°C for 15 minutes, blocked using donkey serum (Jackson Immuno Research, 017-000-121), and stained overnight at 4°C with
Techniques: Expressing, Formalin-fixed Paraffin-Embedded, Staining, Marker
Journal: PLoS ONE
Article Title: Common Human Cancer Genes Discovered by Integrated Gene-Expression Analysis
doi: 10.1371/journal.pone.0001149
Figure Lengend Snippet: Common up-regulated genes in human malignancies
Article Snippet: After blocking in 10% of normal goat serum in PBS, all primary antibodies were incubated overnight at 4° C , including
Techniques:
Journal: PLoS ONE
Article Title: Common Human Cancer Genes Discovered by Integrated Gene-Expression Analysis
doi: 10.1371/journal.pone.0001149
Figure Lengend Snippet: QRT-PCR analysis of selected common cancer genes in initial screens
Article Snippet: After blocking in 10% of normal goat serum in PBS, all primary antibodies were incubated overnight at 4° C , including
Techniques:
Journal: PLoS ONE
Article Title: Common Human Cancer Genes Discovered by Integrated Gene-Expression Analysis
doi: 10.1371/journal.pone.0001149
Figure Lengend Snippet: SPP1 positive staining presents in cytoplasm and nuclear of tumor cells in lung cancer (A, right), breast cancer (B, right), ovary cancer (C, right), and Colon cancer (D, right) while negative staining in normal lung (A, left), and ovary (C, left), weak staining in normal breast (B, left), and colon (D, left).
Article Snippet: After blocking in 10% of normal goat serum in PBS, all primary antibodies were incubated overnight at 4° C , including
Techniques: Staining, Negative Staining